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Digitimer North America LLC electrical pulse stimulation
Fig. 1 Effects of chronic THC treatment on striatal DA release in acute brain slices using FSCV. A Schematic illustrating chronic treatment and timepoints of FSCV recordings in striatal subregions where a carbon fiber recording electrode and stimulating electrode were placed to record <t>electrical</t> <t>stimulation-elicited</t> DA release. Purple rectangles indicate abstinence timepoints used in recordings. B DA release in males (♂) during early abstinence following chronic treatment. No differences detected in DLS (Ftreatment(1,72) = 0.02, p = 0.8712), DMS (Ftreatment(1,84) = 1.01, p = 0.3168), or NAc (Ftreatment(1,84) = 3.07, p = 0.0834). C DA release in males during late abstinence following chronic treatment. No differences detected in DLS (Ftreatment(1,186) = 0.45, p = 0.5019) or NAc (Ftreatment(1,87) = 0.64, p = 0.4270). In DMS THC treated mice had elevated DA release (Ftreatment (1,71) = 13.56; p = 0.0004). D DA release in females (♀) during early abstinence following chronic treatment. DA release was elevated in DLS (Ftreatment(1,72) = 51.61, p = <0.0001), DMS (Ftreatment(1,72) = 33.82, p = <0.0001) and NAc (Ftreatment(1,60) = 11.69, p = 0.0011). E DA release in females during late abstinence following acute treatment. No differences detected in DLS (Ftreatment(1,84) = 2.54, p = 0.1151), DMS (Ftreatment(1,84) = 1.68, p = 0.1981), or NAc (Ftreatment(1,72) = <0.01, p = 0.9976).
Electrical Pulse Stimulation, supplied by Digitimer North America LLC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 1 Effects of chronic THC treatment on striatal DA release in acute brain slices using FSCV. A Schematic illustrating chronic treatment and timepoints of FSCV recordings in striatal subregions where a carbon fiber recording electrode and stimulating electrode were placed to record <t>electrical</t> <t>stimulation-elicited</t> DA release. Purple rectangles indicate abstinence timepoints used in recordings. B DA release in males (♂) during early abstinence following chronic treatment. No differences detected in DLS (Ftreatment(1,72) = 0.02, p = 0.8712), DMS (Ftreatment(1,84) = 1.01, p = 0.3168), or NAc (Ftreatment(1,84) = 3.07, p = 0.0834). C DA release in males during late abstinence following chronic treatment. No differences detected in DLS (Ftreatment(1,186) = 0.45, p = 0.5019) or NAc (Ftreatment(1,87) = 0.64, p = 0.4270). In DMS THC treated mice had elevated DA release (Ftreatment (1,71) = 13.56; p = 0.0004). D DA release in females (♀) during early abstinence following chronic treatment. DA release was elevated in DLS (Ftreatment(1,72) = 51.61, p = <0.0001), DMS (Ftreatment(1,72) = 33.82, p = <0.0001) and NAc (Ftreatment(1,60) = 11.69, p = 0.0011). E DA release in females during late abstinence following acute treatment. No differences detected in DLS (Ftreatment(1,84) = 2.54, p = 0.1151), DMS (Ftreatment(1,84) = 1.68, p = 0.1981), or NAc (Ftreatment(1,72) = <0.01, p = 0.9976).
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Fig. 1 Effects of chronic THC treatment on striatal DA release in acute brain slices using FSCV. A Schematic illustrating chronic treatment and timepoints of FSCV recordings in striatal subregions where a carbon fiber recording electrode and stimulating electrode were placed to record <t>electrical</t> <t>stimulation-elicited</t> DA release. Purple rectangles indicate abstinence timepoints used in recordings. B DA release in males (♂) during early abstinence following chronic treatment. No differences detected in DLS (Ftreatment(1,72) = 0.02, p = 0.8712), DMS (Ftreatment(1,84) = 1.01, p = 0.3168), or NAc (Ftreatment(1,84) = 3.07, p = 0.0834). C DA release in males during late abstinence following chronic treatment. No differences detected in DLS (Ftreatment(1,186) = 0.45, p = 0.5019) or NAc (Ftreatment(1,87) = 0.64, p = 0.4270). In DMS THC treated mice had elevated DA release (Ftreatment (1,71) = 13.56; p = 0.0004). D DA release in females (♀) during early abstinence following chronic treatment. DA release was elevated in DLS (Ftreatment(1,72) = 51.61, p = <0.0001), DMS (Ftreatment(1,72) = 33.82, p = <0.0001) and NAc (Ftreatment(1,60) = 11.69, p = 0.0011). E DA release in females during late abstinence following acute treatment. No differences detected in DLS (Ftreatment(1,84) = 2.54, p = 0.1151), DMS (Ftreatment(1,84) = 1.68, p = 0.1981), or NAc (Ftreatment(1,72) = <0.01, p = 0.9976).
Electrical Pulse Stimulation, supplied by AutoMate Scientific Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 1 Effects of chronic THC treatment on striatal DA release in acute brain slices using FSCV. A Schematic illustrating chronic treatment and timepoints of FSCV recordings in striatal subregions where a carbon fiber recording electrode and stimulating electrode were placed to record <t>electrical</t> <t>stimulation-elicited</t> DA release. Purple rectangles indicate abstinence timepoints used in recordings. B DA release in males (♂) during early abstinence following chronic treatment. No differences detected in DLS (Ftreatment(1,72) = 0.02, p = 0.8712), DMS (Ftreatment(1,84) = 1.01, p = 0.3168), or NAc (Ftreatment(1,84) = 3.07, p = 0.0834). C DA release in males during late abstinence following chronic treatment. No differences detected in DLS (Ftreatment(1,186) = 0.45, p = 0.5019) or NAc (Ftreatment(1,87) = 0.64, p = 0.4270). In DMS THC treated mice had elevated DA release (Ftreatment (1,71) = 13.56; p = 0.0004). D DA release in females (♀) during early abstinence following chronic treatment. DA release was elevated in DLS (Ftreatment(1,72) = 51.61, p = <0.0001), DMS (Ftreatment(1,72) = 33.82, p = <0.0001) and NAc (Ftreatment(1,60) = 11.69, p = 0.0011). E DA release in females during late abstinence following acute treatment. No differences detected in DLS (Ftreatment(1,84) = 2.54, p = 0.1151), DMS (Ftreatment(1,84) = 1.68, p = 0.1981), or NAc (Ftreatment(1,72) = <0.01, p = 0.9976).
Monophasic Rectangular Pulses, supplied by AutoMate Scientific Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 1 Effects of chronic THC treatment on striatal DA release in acute brain slices using FSCV. A Schematic illustrating chronic treatment and timepoints of FSCV recordings in striatal subregions where a carbon fiber recording electrode and stimulating electrode were placed to record <t>electrical</t> <t>stimulation-elicited</t> DA release. Purple rectangles indicate abstinence timepoints used in recordings. B DA release in males (♂) during early abstinence following chronic treatment. No differences detected in DLS (Ftreatment(1,72) = 0.02, p = 0.8712), DMS (Ftreatment(1,84) = 1.01, p = 0.3168), or NAc (Ftreatment(1,84) = 3.07, p = 0.0834). C DA release in males during late abstinence following chronic treatment. No differences detected in DLS (Ftreatment(1,186) = 0.45, p = 0.5019) or NAc (Ftreatment(1,87) = 0.64, p = 0.4270). In DMS THC treated mice had elevated DA release (Ftreatment (1,71) = 13.56; p = 0.0004). D DA release in females (♀) during early abstinence following chronic treatment. DA release was elevated in DLS (Ftreatment(1,72) = 51.61, p = <0.0001), DMS (Ftreatment(1,72) = 33.82, p = <0.0001) and NAc (Ftreatment(1,60) = 11.69, p = 0.0011). E DA release in females during late abstinence following acute treatment. No differences detected in DLS (Ftreatment(1,84) = 2.54, p = 0.1151), DMS (Ftreatment(1,84) = 1.68, p = 0.1981), or NAc (Ftreatment(1,72) = <0.01, p = 0.9976).
Compex Motion Stimulator, supplied by Compex Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 1 Effects of chronic THC treatment on striatal DA release in acute brain slices using FSCV. A Schematic illustrating chronic treatment and timepoints of FSCV recordings in striatal subregions where a carbon fiber recording electrode and stimulating electrode were placed to record electrical stimulation-elicited DA release. Purple rectangles indicate abstinence timepoints used in recordings. B DA release in males (♂) during early abstinence following chronic treatment. No differences detected in DLS (Ftreatment(1,72) = 0.02, p = 0.8712), DMS (Ftreatment(1,84) = 1.01, p = 0.3168), or NAc (Ftreatment(1,84) = 3.07, p = 0.0834). C DA release in males during late abstinence following chronic treatment. No differences detected in DLS (Ftreatment(1,186) = 0.45, p = 0.5019) or NAc (Ftreatment(1,87) = 0.64, p = 0.4270). In DMS THC treated mice had elevated DA release (Ftreatment (1,71) = 13.56; p = 0.0004). D DA release in females (♀) during early abstinence following chronic treatment. DA release was elevated in DLS (Ftreatment(1,72) = 51.61, p = <0.0001), DMS (Ftreatment(1,72) = 33.82, p = <0.0001) and NAc (Ftreatment(1,60) = 11.69, p = 0.0011). E DA release in females during late abstinence following acute treatment. No differences detected in DLS (Ftreatment(1,84) = 2.54, p = 0.1151), DMS (Ftreatment(1,84) = 1.68, p = 0.1981), or NAc (Ftreatment(1,72) = <0.01, p = 0.9976).

Journal: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology

Article Title: Changes in striatal dopamine release, sleep, and behavior during spontaneous Δ-9-tetrahydrocannabinol abstinence in male and female mice.

doi: 10.1038/s41386-022-01326-0

Figure Lengend Snippet: Fig. 1 Effects of chronic THC treatment on striatal DA release in acute brain slices using FSCV. A Schematic illustrating chronic treatment and timepoints of FSCV recordings in striatal subregions where a carbon fiber recording electrode and stimulating electrode were placed to record electrical stimulation-elicited DA release. Purple rectangles indicate abstinence timepoints used in recordings. B DA release in males (♂) during early abstinence following chronic treatment. No differences detected in DLS (Ftreatment(1,72) = 0.02, p = 0.8712), DMS (Ftreatment(1,84) = 1.01, p = 0.3168), or NAc (Ftreatment(1,84) = 3.07, p = 0.0834). C DA release in males during late abstinence following chronic treatment. No differences detected in DLS (Ftreatment(1,186) = 0.45, p = 0.5019) or NAc (Ftreatment(1,87) = 0.64, p = 0.4270). In DMS THC treated mice had elevated DA release (Ftreatment (1,71) = 13.56; p = 0.0004). D DA release in females (♀) during early abstinence following chronic treatment. DA release was elevated in DLS (Ftreatment(1,72) = 51.61, p = <0.0001), DMS (Ftreatment(1,72) = 33.82, p = <0.0001) and NAc (Ftreatment(1,60) = 11.69, p = 0.0011). E DA release in females during late abstinence following acute treatment. No differences detected in DLS (Ftreatment(1,84) = 2.54, p = 0.1151), DMS (Ftreatment(1,84) = 1.68, p = 0.1981), or NAc (Ftreatment(1,72) = <0.01, p = 0.9976).

Article Snippet: DA release was evoked by rectangular, electrical pulse stimulation (250–800 μA; 2 ms, monophasic) applied every 5min with a NL 800 A Current Stimulus Isolator (Digitimer, Hertfordshire, UK).

Techniques: